U-025

ACAPELLA, A capillary-based submicroliter automated sample preparation system for genome analysis

Authors: Deirdre R. Meldrum*, Harold T. Evensen*, William H. Pence**, Stephen E. Moody**, David L. Cunningham**, and Peter J. Wiktor***
Affiliation: * Department of Electrical Engineering, Genomation Laboratory, University of Washington, Box 352500, Seattle, WA 98195-2500

** Orca Photonic Systems, Inc., 14662 NE 95th Street, Redmond, WA 98052

*** Engineering Arts, 3226 NE 87th Street, Seattle, WA 98115

Abstract
The Genomation Laboratory in the Electrical Engineering Department at the University of Washington has been developing an automated, high-throughput submicroliter-scale reaction preparation system for use in molecular biology, especially as part of the Human Genome Project and other high-throughput DNA sequencing endeavors. Small glass capillaries enable the preparation, handling and monitoring of one-microliter reaction volumes. The Genomation Laboratory, with corporate partners Orca Photonic Systems, Inc. and Engineering Arts, has developed modules for aspiration, dispensing, mixing, transport and rapid thermal treatment of biological samples contained in glass capillaries. The "ACAPELLA 1K" is the first integration of these modules, designed to process 1,000 samples in an 8-hour day. It has served as a test bed for the technologies as well as for performing biological experiments in conjunction with the University of Washington Genome Center. The Genomation Laboratory is presently developing the next-stage "ACAPELLA 5K" system based on the results of the ACAPELLA 1K system.

Deirdre Meldrum
Department of Electrical Engineering, Genomation Laboratory
University of Washington, Box 352500, Seattle, WA 98195-2500
deedee@isdl.ee.washington.edu